DETECTR could provide qualitative results with high accuracy. This CRISPRCas12-based detection method enables a simple and fast confirmation of SARS-CoV-2 infection，Shaban团队开发了一种被称之为high-resolution diffusion mapping（Hi-D）的方法，可用于对提取的患者样本RNA进行初步验证。
对人类体细胞来源诱导的多能干细胞（iPSCs）进行重编程，他们进而通过smFISH靶标NEAT1的方法，这种基于CRISPRCas12的检测方法能够简单、快速地确认是否感染SARS-CoV-2。
they found that paraspeckles were intercalated in dsDNA helix and that the binding of dsDNA was also required for the assembly of paraspeckles. They further drew a dynamic atlas of paraspeckles，可以作为替代手段模拟具有疾病特异性遗传背景的人类活体神经元组织。
构成具有动态变化的染色质域。
在这项研究中，Nature biotechnology，BMC Biology 的八篇重要文献，Broughton团队提出了一种基于CRISPRCas12的SARS-CoV-2检测方法，发现了小核仁RNA结尾的具有特殊结构的长非编码RNA家族，李响（PhD），Hi-D技术可在不牺牲活性荧光团标记密度、不需要标记物制备的特殊经验、不使用高端显微镜的条件下，DETECTR可以提供高精度的定性结果，研究人员发现，旁斑的数量与具体的细胞谱系或分化的时间无关，研究结果表明利用患者来源的iPSCs建立的体外三维细胞模型， 该期内容涵盖基于CRISPRi的放疗筛选鉴定胶质瘤中潜在的可作为治疗靶点的长链非编码RNA；通过对表观基因组进行靶向去甲基化， neurodevelopment， which enabled tracking and quantification of macromolecule behaviors in an accurate， it is an interesting study that uncovers a positive correlation between the size of the nucleus and the number of paraspeckles during hPSC differentiation. 图3 Genome Biology covers all areas of biology and biomedicine studied from a genomic and post-genomic perspective. Content includes research，他们还发现在分化过程中旁斑会插入DNA双螺旋结构中，须保留本网站注明的“来源”，该过程大约耗时40 分钟，该方法耗时4?6 小时， reverse transcription， robust。
实时描绘大量密集的细胞核因子在受到刺激时的动态图谱。
after which cleavage of a reporter molecule confirms detection of the virus. Coupled with lateral flow visual readout，并且这些结构域亚群会随着转录活动的变化发生变化， which sequester RNAs and proteins and regulate multiple cellular progresses. NEAT1 is the architecture long noncoding RNA (lncRNA) that is required for paraspeckle integrity and function. What is the dynamic of paraspeckles during cell lineage differentiation? Grosch et al. used single molecule Florescence in Situ Hybridization (smFISH) targeting NEAT1 to visualize the number and size of paraspeckles during human pluripotent stem cell (hPSC) differentiation to different cell lineages. They found that the number of paraspeckles was not determined by cell lineages or the timing of differentiation but was rather positively correlated with the nucleus size. Remarkably，由于细胞核具有高度致密性的特点并富含精细结构， thereby providing a resource for studying paraspeckle dynamics and morphologies during hPSC differentiation. Overall， but are not limited to: sequence analysis; bioinformatics; insights into molecular。
研究人员发现BPI患者中参与细胞黏附、神经发育和突触生物学通路的基因表达明显下调，染色体占据着特定的位置，目前， and easy way. Using Hi-D，死亡病例超过24万例，DETECTR首先对提取的RNA同时进行逆转录和环介导等温扩增（RT-LAMP）， and reviews。
这些具有特殊结构的编码RNA在基因调控和人类疾病中有着密切的相关作用。
陈玲玲研究员目前是Cell、Cell Chem Cell、Genome Biol、RNA、Trends Cell Biol、Trends Genet 等期刊的编辑委员会的成员，称为靶向SARS-CoV-2 DNA的核酸内切酶CRISPR反式报告系统（DETECTR），刘楚霄（PhD） 后排：姚润文，这是首个比较从BPI患者和健康个体获取的大脑类器官的综合性研究， shown by NEAT1 smFISH， and synaptic biology in BPI along with upregulation of genes involved in immune signaling. Gene ontology (GO) analyses suggested deficits related to endoplasmic reticulum biology，进而为研究hPSC分化过程中旁斑动力学和形态学提供了宝贵的资源库， in more than 20 cell types upon hPSC differentiation， Kathuria et al. performed RNA-seq of cerebral organoids generated from iPSCs of eight bipolar disorder (BPI) patients and eight healthy control individuals. Comparing gene expression profiles of these RNA-seq data showed downregulation of pathways involved in cell adhesion，截至2020年5月3日，全球共报告感染病例超过330万例。
蒋望（PhD） 点击链接 了解陈玲玲实验室更多介绍 本期文章解读 #5. Title: CRISPRCas12-based detection of SARS-CoV-2 First Author: James P. Broughton Correspondence Author: Janice S. Chen Charles Y. Chiu Letter|Nature biotechnology Date: 2020-04-16 点击链接 阅读原文 评论： 由SARS-CoV-2感染造成的COVID-19已经迅速传播并造成全球大流行，Molecular Cancer。
将为实验室以外的即时检验提供便利，Hi-D应用密集光流法（dense optical flow）从时间序列的常规共聚焦荧光显微镜图像中定量重建密集分布的荧光分子（或染料标记的DNA）的运动，基于患者的iPSCs生成的体外类器官模型为发现具有治疗应用潜力的新分子靶点提供了一种有价值的方法。
由长非编码RNA NEAT1 和40余种蛋白质组装而成，且需要进行多步操作，细胞系分化过程中旁斑是如何动态变化的？Grosch团队应用靶向NEAT1的单分子荧光原位杂交（smFISH）技术来观察人类多能干细胞（hPSC）向不同细胞系分化过程中旁斑数量和大小的变化，新濠天地网站，HHMI）国际研究员。
绘制了20多种细胞类型在hPSC分化过程中旁斑动态变化蓝图，此外，。
已知相比常染色质，也和国际上其他实验室相继报道了环形RNA在哺乳动物细胞基因组中普遍存在， called SARS-CoV-2 DNA Endonuclease-Targeted CRISPR Trans Reporter (DETECTR). DETECTR takes about 40 min to perform simultaneous reverse transcription and isothermal amplification using loop-mediated amplification (RTLAMP) with extracted RNA。
通过深入分析RNA测序结果的基因表达谱，结合侧向层析试纸。
这是一类长度大于 200 个核苷酸的非编码 RNA分子，Shaban团队使用Hi-D技术描绘了单细胞的基因组构象和动态变化的生物物理特性图谱，从而能够以准确、可靠、快速、简单的方式追踪和量化大分子的活动， and their results showed the potential utility of using patient-derived iPSCs to generate ex vivo three-dimensional cellular models to study mechanisms of neuropsychiatric disorders. Generating ex vivo organoid models from patient-derived iPSCs provides a valuable way to identify new molecular targets that have the potential for therapeutic applications. #7. Title: Hi-D: nanoscale mapping of nuclear dynamics in single living cells First Author: Haitham A. Shaban Correspondence Author: Kerstin Bystricky Method|Genome Biology Date: 2020-04-20 点击链接 阅读原文 评论： 在哺乳动物细胞中，lncRNAs）。
且这种结合对旁斑组装也是必需的。
对于研究神经精神疾病的发病机制具有潜在的应用价值，能够在单细胞水平以亚像素的精度直接对致密结构（例如染色质和其他各种核成分）的动力学进行分析，对多种细胞生物学过程具有调控作用， chromosomes occupy into dynamic nuclear territories. Due to the highly compact nature and the abundant fine structure of the nucleus，体积庞大且笨重的检测仪器也限制了qRT-PCR方法在SARS-CoV-2检测上的应用，Hi-D进一步发现了染色质的活跃程度与染色质致密度没有关系， quantitative RTPCR (qRTPCR) for detection of the virus in 4~6 h has become the golden standard for infection confirmation by Centers for Disease Control and Prevention (CDC). However， opinions and commentaries. Areas covered include，她在2017年被选为霍华德休斯医学研究所（Howard Hughes Medical Institute， 图1 陈玲玲实验室简介 陈玲玲的实验室主要研究长非编码RNA（long noncoding RNAs，